Conventional Test Method:

Lethal challenge test in the batch potency testing of inactivated swine erysipelas vaccines (Monograph 064, PhEur)

Alternative Test Method:

Name & Description:

Serological Method (ELISA) for the Batch Potency Testing of Inactivated Swine Erysipelas Vaccines

This test uses an alkaline extract of E. rhusiopathiae as the coating antigen to detect protective serum antibodies of immunized mice.  Serum antibody levels are estimated by comparing the titration curves of the test serum and reference serum.

Validation Status:

EU: Validated by ECVAM (2002)

Regulatory Status:

EU: Included in PhEur monograph: swine erysipelas vaccine (4.6; No 01/2004:0064)

Effect or Potential Effect on Animal Use:


(Does not rely on death as an endpoint)

Information Last Reviewed:
May 2012