Conventional Test Method:

No specific conventional test method is superseded

Alternative Test Method:

Name & Description:

In Vitro Comet Assay (Single-Cell Gel Electrophoresis [SCGE] Technique)

Cells in culture are treated with the test compound. The cells are then lysed and their DNA is run on a gel. If the DNA is damaged, the migrated pieces can be detected through staining.

Validation Status:

Japan: JaCVAM presently conducting validation 

Regulatory Status:

OECD: new Test Guideline proposed

Effect or Potential Effect on Animal Use:

(part of a tiered testing strategy that results in use of  fewer animals)

Information Last Reviewed:
May 2012