Conventional Test Method:

Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)

Alternative Test Method:

Name & Description:

Slug Mucosal Irritation (SMI) Assay

Slugs are exposed to the test compound then assessed for amount of mucus produced, as well as concentration of proteins and enzymes released from the slugs’ body wall.

Validation Status:

EU: Prevalidation study results have been submitted to ECVAM for evaluation.

Regulatory Status:

No information

Effect or Potential Effect on Animal Use:


  • Adriaens E. (2006) The slug mucosal irritation assay: an alternative assay for local tolerance testing. National Centre for the Replacement, Refinement and Reduction of Animals in Research.
  • Adriaens E., Bytheway H., De Wever B., Eschrich D., Guest R., Hansen E., Vanparys P., Schoeters G., Warren N., Weltens R., Whittingham A. and Remon J.P. (2008) Successful prevalidation of the slug mucosal irritation test to assess the eye irritation potency of chemicals. Toxicology in Vitro 22:1285-1296.
Information Last Reviewed:
May 2012