Conventional Test Method:

Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)

Alternative Test Method:

Name & Description:

Isolated Rabbit Eye (IRE) Test Method

Exposes isolated rabbit eyes (obtained from animals previously euthanized for research or those commercially sacrificed) to test substance and evaluates corneal swelling, corneal opacity, fluorescein retention, and effects on the corneal epithelium.

Validation Status:

EU: Concluded not sufficiently validated by ESAC (2007)

US: Concluded not sufficiently validated by ICCVAM (2007)

Regulatory Status:

EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)

Effect or Potential Effect on Animal Use:

(Substances identified as irritants by test will not progress to animal testing)

Information Last Reviewed:
May 2012