Conventional Test Method:

Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)

Alternative Test Method:

Name & Description:

Isolated Chicken Eye (ICE) Test Method

Exposes isolated chicken eyes (obtained from slaughterhouses) to the test substance and evaluates corneal swelling, corneal opacity, and fluorescein retention to determine the irritation or corrosion potential of a substance.

Validation Status:

OECD: validated

EU: Endorsed as valid by ESAC (2007)

US: Endorsed as valid by ICCVAM (2007)

Regulatory Status:

OECD: TG 438 (2013)

EU: Accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)

US: Accepted as a screening test to detect ocular corrosives and severe irritants in a tiered testing strategy (2008)

Effect or Potential Effect on Animal Use:

(Substances identified as irritants by test will not progress to animal testing)

Information Last Reviewed:
June 2014