Conventional Test Method:

Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)

Alternative Test Method:

Name & Description:

Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) Test Method

Exposes the chorionic membrane of embryonated chicken eggs to test compound and evaluates acute effects on the small blood vessels and proteins of this soft tissue membrane to identify materials that are severely irritating to the eye. The test method is based on the observation that the CAM of an embryonated hen’s egg is similar to the vascularized mucosal tissues of the eye.

Validation Status:

EU: Concluded not sufficiently validated by ESAC (2007)

US: Concluded not sufficiently validated by ICCVAM (2007)

Regulatory Status:

EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)

Effect or Potential Effect on Animal Use:

(Substances identified as irritants by test will not progress to animal testing)

Information Last Reviewed:
May 2012