Three Rs Microsite

Three Rs Searches
& Animal Index

Questions to assess if your Three Rs search is complete

To assist in deciding if your Three Rs search is complete, it may be useful to review the following questions:

  • Is the proposed experiment or test duplicative?
  • Are there any in vitro techniques that could replace use of animals?
  • Have any computer simulations been developed that relate to the study?
  • Are there any alternative animal models of lower sentience?
  • Is there useful and current information about the proposed animal model?
  • Has a particular strain of animal been shown to be more sensitive to the effects that will be studied?
  • Is there information on the proposed model that might allow the use of fewer animals or might reduce any pain experienced by the animals?
  • Could in vitro methods be incorporated into the protocol in any way to reduce the number of animals used (e.g., for early screening)?
  • Have any statistical models been developed for use in this type of study, and would these affect the design of the experiment?
  • Is there a way to decrease the level of invasiveness of the protocol without compromising the scientific objective?
  • Could the proposed anesthetics, analgesics or other drugs pose a confounding influence on the experimental outcome?
  • Is there information about assessing welfare and the level of pain of the animals?

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