Conventional Test Method:

Residual Toxicity in Diptheria Vaccine (Guinea pig)

Alternative Test Method:

Name & Description:

Vero Cell Test of Diphtheria Toxoid Vaccines

Vero (African green monkey kidney) cells and MTT dye is added to wells containing either bacterial culture filtrates or reference diphtheria toxin. Vero cell viability is measured by observing the absorbance at 570 nm.

Validation Status:

No information

Regulatory Status:

EU: Accepted into PhEur

Effect or Potential Effect on Animal Use:

Replacement

References:
  • Council of Europe (CE) (2008) Absence of toxin and irreversibility of toxoid - Diphtheria vaccine (adsorbed). In: European Pharmacopoeia, 6th ed. Strasbourg, France: CE.
Information Last Reviewed:
May 2012