Conventional Test Method:

No specific conventional test method is superseded

Alternative Test Method:

Name & Description:

Saacharomyces cerevisiae Mitotic Recombination Assay

This assay measures mitotic recombination (gene conversion or crossing-over) in yeast. Crossing-over is generally assayed by the production of recessive homozygous colonies or sectors produced in a heterozygous strain, whereas gene conversion is assayed by the production of prototrophic revertants produced in an auxotrophic heteroallelic strain carrying two different defective alleles of the same gene. Stationary or growing cells are exposed to the test compound with and without an exogenous mammalian metabolic activation system.  After incubation, plates are scored for survival and the induction of mitotic recombination.

Validation Status:

No information

Regulatory Status:

OECD: TG 481 (1986)

Effect or Potential Effect on Animal Use:

Reduction
(part of a tiered testing strategy that results in use of  fewer animals)

References:
Information Last Reviewed:
May 2012