Conventional Test Method:

No specific conventional test method is superseded

Alternative Test Method:

Name & Description:

In Vitro Unscheduled DNA Synthesis  (UDS) in Mammalian Cells Test

This procedure utilizes primary cultures, human lymphocytes or established cell lines to detect DNA repair synthesis after excision and removal of a stretch of DNA containing the region of damage induced by chemical or physical agents. The test incorporates tritium-labelled thymidine (3H-TdR) into the DNA of mammalian cells which are not in the S-phase of the cell cycle. The uptake of 3H-TdR may be determined by autoradiography or by liquid scintillation counting (LSC) of DNA from the treated cells. Mammalian cells are treated with the test compound, with and without exogenous mammalian metabolic activation.

Validation Status:

No information

Regulatory Status:

OECD: TG 482 (1986)

Effect or Potential Effect on Animal Use:

Reduction
(part of a tiered testing strategy that results in use of  fewer animals)

References:
Information Last Reviewed:
May 2012