Conventional Test Method:

No specific conventional test method is superseded

Alternative Test Method:

Name & Description:

Bacterial Reverse Mutation Test (Ames test)

This test uses amino acid requiring bacteria of at least five strains of Salmonella typhimurium and Escherichia coli to detect point mutations. Bacterial cells are plated on agar lacking an amino acid required by the test strain, and then exposed to the test compound in the presence and in the absence of an exogenous metabolic activation system. The principle of this test is that it detects new mutations which revert mutations already present in the test strains, and restore the functional capability of the bacteria to synthesize an essential amino acid. The revertant bacteria are detected by their ability to grow in the absence of the amino acid required by the parent test strain. Lack of bacterial growth may indicate that test compound is non-mutagenic.

Validation Status:

No information

Regulatory Status:

OECD: TG 471 (1997)

US: Approved for the safety testing of food by US FDA (2000)

Effect or Potential Effect on Animal Use:

Reduction
(part of a tiered testing strategy that results in use of  fewer animals)

References:
Information Last Reviewed:
May 2012