Conventional Test Method:

Mouse Bioassay (PSP)
(Association of Official Analytical Chemists [AOAC] Official Method 959.08)

Alternative Test Method:

Name & Description:

Pre-column HPLC Method (Lawrence method)

Acetic acid shellfish extracts are prepared and oxidized before being analyzed using HPLC. Samples may require two runs. 

Validation Status:

Association of Analytical Communities (AOAC): validated through collaborative trial (2005)

EU: Inter-laboratory validation study using AOAC official method 2005.06 organized by the Community Reference Laboratory of Marine Biotoxins (CRLMB) concluded that the test can be useful as a screening method but needs further work to implement it for Official PSP Control and as a Reference Method at the EU (2007)

Regulatory Status:

AOAC Official Method 2005.06

FAO/WHO: accepted as a screening test (Codex Type IV method [Tentative Method of Analysis]  but could be promoted to Type III [Alternative Approved Method of Analysis] through further validation studies)

Effect or Potential Effect on Animal Use:

Replacement

References:
  • AOAC International (2005) AOAC Official Method 2005.06; Paralytic shellfish poisoning toxins in shellfish: Prechromatographic oxidation and liquid chromatography with fluorescence detection, First Action 2005. AOAC International, Gaithersburg.
  • Ben-Gigirey B., Rodríguez-Velasco M.L., Villar-González A. & Botana L.M. (2007) Influence of the sample toxic profile on the suitability of a high performance liquid chromatography method for official paralytic shellfish toxins control. Journal of Chromatography A 1140: 78-87.
  • Lawrence J.F. & Niedzwiadek B. (2001) Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection. Journal of AOAC International 84: 1099-1108.
  • Lawrence J.F., Niedzwiadek B. & Menard C. (2005) Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study. Journal of AOAC International 88: 1714-1732.
Information Last Reviewed:
May 2012