Conventional Test Method:

Mouse Bioassay (PSP)
(Association of Official Analytical Chemists [AOAC] Official Method 959.08)

Alternative Test Method:

Name & Description:

Jellett Rapid Test (formerly MIST AlertTM test)

This test uses polyclonal PSP toxin antibodies and is based on the principles of lateral flow immunochromatography. Shellfish extracts are prepared using the 10 g mini AOAC extraction method. The extract is mixed with buffer and the resulting solution is applied to a MIST Alertä test cassette. If the test is positive for the toxin, a single line appears in the window. If the test is negative, two lines appear in the window.

Validation Status:

Not formally validated

Regulatory Status:

FAO/WHO: accepted as a screening test (it is not accepted as a stand-alone test because it is a qualitative method) (Codex Tentative Method of Analysis, Type IV) (2004) 

US: Accepted by the USDA for use by the US National Shellfish Sanitation Program (2004)

Effect or Potential Effect on Animal Use:

Reduction
(Reduction of animal use by up to 30%)

References:
  • FAO/IOC/WHO (2004) Report of the joint FAO/IOC/WHO ad hoc expert consultation on biotoxins in bivalve molluscs. Oslo, Norway, September 26-30.
  • Jellett J.F., Roberts R.L., Laycock M.V., Quilliam M.A. and Barrett R.E. (2002) Detection of paralytic shellfish poisoning (PSP) toxins in shellfish tissue using MIST Alert, a new rapid test, in parallel with the regulatory AOAC mouse bioassay. Toxicon 40(10):1407-1425.
  • Mackintosh F.H., Gallacher S., Shanks A.M. and Smith E.A. (2002) Assessment of MIST Alert, a commercial qualitative assay for detection of paralytic shellfish poisoning toxins in bivalve molluscs. Journal of AOAC International 85(3):632-641.
  • Oshiro M., Pham L., Csuti D., Dodd M., Inami G.B. and Brenden R.A. (2006) Paralytic shellfish poisoning surveillance in California using the Jellett Rapid PSP test. Harmful Algae 5(1):69-73.
Information Last Reviewed:
May 2012