Conventional Test Method:
Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)
Alternative Test Method:
- Name & Description:
Fluorescein Leakage Test
a cytotoxicity and cell-function based in vitro assay that is performed on a confluent monolayer of CB997 tubular epithelial cells that are grown on semi-permeable inserts and model the non-proliferating state of the in vivo corneal epithelium.
- Validation Status:
EU: Endorsed as valid by ESAC (2009).
- Regulatory Status:
OECD: TG 460 (2012)
- Effect or Potential Effect on Animal Use:
(Substances identified as irritants by test will not progress to animal testing)
- Information Last Reviewed:
- May 2012