Conventional Test Method:

Acute Eye Irritation/Corrosion (Draize rabbit eye irritation test) (OECD TG 405)

Alternative Test Method:

Name & Description:

Cytosensor Microphysiometer (CM) modified

The CM test method is a cytotoxicity and cell-function based in vitro assay that is performed on a sub-confluent monolayer of adherent mouse L929 fibroblasts cultured on a transwell polycarbonate insert with a porous membrane, which functions as an electrode, and a light-addressable potentiometric sensor detecting changes in pH (acidity)

Validation Status:

EU: Endorsed as valid by ESAC (2009)

US: can be used as a screening test to identify some types of substances that may cause permanent or severe eye injuries (2010).

Regulatory Status:

OECD: Draft new TG (2011)

Effect or Potential Effect on Animal Use:

Reduction 
(Substances identified as irritants by test will not progress to animal testing)

References:
Information Last Reviewed:
May 2012