Eye Irritation/Corrosion

Conventional
test method

Alternative Test Method

Last Reviewed
Name & Description Validation Status Regulatory Status Effect or Potential Effect on Animal Use
Acute Eye Irritation/Corrosion
(Draize rabbit eye irritation test)
(OECD TG 405)
Bovine Corneal Opacity and Permeability (BCOP) Test Method
Description and references
OECD: validated

EU: Endorsed as valid by ESAC (2007)

US: Endorsed as valid by ICCVAM (2007)
OECD: TG 437 (2013)

EU: Accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)

US: Accepted as a screening test to detect ocular corrosives and severe irritants in a tiered testing strategy (2008)
Reduction
(Substances identified as irritants by test will not progress to animal testing)
June 2014
Isolated Chicken Eye (ICE) Test Method
Description and references
EU: validated

US: Endorsed as valid by ICCVAM (2007)
OECD: TG 438 (2013)

EU: Accepted for use to positively identify and label substances as severe eye irritants (R41) (2002)

US: Accepted as a screening test to detect ocular corrosives and severe irritants in a tiered testing strategy (2008)
Reduction
(Substances identified as irritants by test will not progress to animal testing)
June 2014
Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) Test Method
Description and references
EU: Concluded not sufficiently validated by ESAC (2007)

US:
Concluded not sufficiently validated by ICCVAM (2007)
EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41) (2002) Reduction
(Substances identified as irritants by test will not progress to animal testing)
May 2012
Isolated Rabbit Eye (IRE) Test Method
Description and references
EU: Concluded not sufficiently validated by ESAC (2007)

US: Concluded not sufficiently validated by ICCVAM (2007)
EU: Although not formally validated, accepted for use to positively identify and label substances as severe eye irritants (R41)(2002) Reduction
(Substances identified as irritants by test will not progress to animal testing)
May 2012
Slug Mucosal Irritation (SMI) Assay
Description and references
EU: Prevalidation study results have been submitted to ECVAM for evaluation. No information Replacement May 2012
Fluorescein Leakage Test
Description and references
EU: Endorsed as valid by ESAC (2009) OECD: OECD TG 460 (2012) Redection
(Substances identified as irritants by test will not progress to animal testing)
October 2012
Cytosensor Microphysiometer (CM) modified
Description and references
EU: Endorsed as valid by ESAC (2009)

US: can be used as a screening test to identify some types of substances that may cause permanent or severe eye injuries (2010)
OECD: Draft new TG (2011) Redection
(Substances identified as irritants by test will not progress to animal testing)
May 2012

 

List of Abbreviations

Test Methods Categories

Toxicity
Dermal Tests
Vaccines