Conventional Test Method:

Acute Mammalian Skin Toxicity

Alternative Test Method:

Name & Description:

Fixed Dose Procedure

Groups of animals (of a single sex) are exposed to the test substance in a stepwise procedure using the appropriate fixed doses. The initial dose level is selected on the basis of a sighting study at the concentration expected to produce clear signs of toxicity without causing severe toxic effects or mortality. Additional animals may be tested at higher or lower fixed doses, depending on the presence or absence of signs of toxicity or mortality. This procedure continues until i) the concentration causing evident toxicity or no more than one death is identified; ii), or when no effects are seen at the highest concentration; or iii) or when deaths occur at the lowest concentration.

Validation Status:

No information

Regulatory Status:

OECD: Draft TG 434 (2004); currently further development of this TG is on hold.

Effect or Potential Effect on Animal Use:

Reduction

Refinement
(Does not rely on death as an endpoint)

References:
Information Last Reviewed:
May 2012