Conventional Test Method:

Acute Inhalation Toxicity (OECD TG 403)

Alternative Test Method:

Name & Description:

Fixed Concentration Procedure

Animals are exposed to the test substance in a stepwise procedure using the appropriate fixed concentrations for vapours, dusts/mists or gases. Initial concentration levels are selected on the basis of a sighting study at the concentration expected to produce clear signs of toxicity without causing severe toxic effects or mortality. Further animals may be tested at higher or lower fixed concentrations, depending on the presence or absence of signs of toxicity or mortality. This procedure continues until i) the concentration causing evident toxicity or no more than one death is Identified; ii), or when no effects are seen at the highest concentration; or iii) or when deaths occur at the lowest concentration.

Validation Status:

No information

Regulatory Status:

OECD: Draft TG 433 (2004); currently further development of this TG is on hold

Effect or Potential Effect on Animal Use:

Refinement
(Does not rely on death as an endpoint)

References:
Information Last Reviewed:
May 2012