Conventional Test Method:

Acute dermal irritation/corrosion (In Vivo Rabbit Skin Corrosivity Test) (OECD TG 404)

Alternative Test Method:

Name & Description:

Corrositex ®

Corrosion refers to the production of irreversible damage to the skin, manifested as visible necrosis through the epidermis and into the dermis. This test system is designed to respond to corrosive substances in a manner similar to animal skin in situ and is composed of a synthetic membrane barrier (bio-barrier) and a Chemical Detection System (CDS). The test substance is applied to the bio-barrier and evaluated for corrosivity based on the length of time it takes to penetrate the bio-barrier, as indicated by the production of  a color change in the underlying liquid Chemical Detection System.  This time is compared to reference times on the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (GHS) classification chart to determine corrosivity/noncorrosivity.

Validation Status:

EU: Endorsed as valid by ECVAM for
testing acids, bases and their derivatives (2000)

US: Corrositex® endorsed as valid by ICCVAM as a stand-alone assay for evaluating the corrosivity or noncorrosivity of acids, bases, and acid derivatives. For other chemical and product classes Corrositex is valid for use as part of a tiered assessment strategy (1999)

Regulatory Status:

OECD: TG 435 (2006)

US: Accepted as an alternative to a procedure specified in the U.S. Department of Transportation (DOT) Hazardous Materials Regulations (DOT-SP 10904) for DOT packing group classification of corrosive materials (1999)

Effect or Potential Effect on Animal Use:

Replacement
(for  testing acids and bases)

OR

Reduction
(Other substances identified as corrosive will not progress to animal tests)

References:
Information Last Reviewed:
May 2012