Conventional Test Method:

Skin Absorption: In Vivo Method (OECD TG 427)

Alternative Test Method:

Name & Description:

Skin Absorption: In Vitro Method

The test substance is applied to the surface of an excised skin sample which sits between two chambers (a donor chamber and a receptor chamber) of a diffusion cell. The receptor fluid is sampled at various time points and analysed for the test chemical and/or metabolites. Analysis of material remaining in the skin layers and washed off the skin allows for further data evaluation. Skin from human or animal sources can be used. Although viable skin is preferred, non-viable skin can also be used. 

Validation Status:

OECD: Endorsed as equivalent to validated (2002)

Regulatory Status:

OECD: TG 428

EU: Regulatory accepted as dermal percutaneous absorption test for the REACH program in place of in vivo animal studies (2008)

Effect or Potential Effect on Animal Use:

Replacement
(When human skin is used)

OR

Reduction and Refinement
(When skin from humanely killed animals is used)

References:
Information Last Reviewed:
May 2012