Three Rs Microsite

Your link to information on replacement,
reduction and refinement alternatives
for animal use in science

Three Rs The Three Rs

Replacing animals in science.

Reducing the number of animals used

Refining care and procedures to minimize pain and distress

Learn more
Upcoming EventsUpcoming Events

Asian Congress 2016 on Alternatives and Animal Use in the Life Science

Karatsu, Saga, and Fukuoka, Japan
November 15-18, 2016

European Commission Scientific Conference: "Non-Animal Approaches - The Way Forward"

Brussels, Belgium
December 6-7, 2016

UFAW 2017 International Symposium. ‘Measuring animal welfare and applying scientific advances – Why is it still so difficult?’

Surrey, UK
June 27-29, 2017

10th World Congress on Alternatives and Animal Use in the Life Science

The 3Rs in Action
Seattle, WA
August 20-24, 2017

News & Publications

September 26, 2016

Efficient Breeding of Genetically Engineered Animals

The Home Office, responsible for overseeing animal-based research in the UK has developed an Assessment Framework to assist institutions consider how best to manage efficient breeding of genetically-engineered animals.

September 19, 2016

The Design of Animal Experiments: Reducing the use of animals in research through better experimental design

The Second Edition of this publication on experimental design is now available. Revised by the original authors, Michael Festing et al., The Design of Animal Experiments is intended for all research scientists who use laboratory animals, with the aim of helping them to design their own experiments more effectively and/or to improve their ability to communicate with professional statisticians when necessary.

September 12, 2016

A Good Death?
Report of the Second Newcastle Meeting on Laboratory Animal Euthanasia

In 2013, the NC3Rs and Newcastle University co-hosted a workshop on the welfare implications of different euthanasia methods for mice, rats and zebrafish. The recently published report presents a summary of findings presented at the meeting, an overview of the current (2016) understanding of the welfare impact of various euthanasia methods, along with recommendations to help inform good practice.

August 29, 2016 revised table of Validated and Accepted Alternative Methods

AltTox maintains a table of alternative methods similar to the CCAC Three Rs Microsite Alternative Test Methods Table. Both tables provide information about the alternative tests and whether regulatory agencies will accept data resulting from the use of these tests.

August 22, 2016

Head mask for cancer patients adapted as a refinement for primate research

A study published recently in the Journal of Neuroscience Methods describes a new Non-invasive Head Immobilization System (NHIS) as an alternative to surgery for macaque monkeys used in neurobiology research.

Recent publications:

News Archive
Three Rs Searches Three Rs

Three Rs Search Guide
Step-by-step strategy for Three Rs information searches

Animal Use Protocol Worksheet
Tool to organize Three Rs information for protocols

CCAC Reference Database
Database of scientific literature used in CCAC publications

Systematic Review
A method of synthesizing scientific evidence

Animal Index
Three Rs information organized by species

Testing Testing

Alternative Test Methods Table
Validated alternatives to conventional regulatory test methods