Three Rs Microsite

Your link to information on replacement,
reduction and refinement alternatives
for animal use in science


Three Rs The Three Rs

Replacing animals in science.

Reducing the number of animals used

Refining care and procedures to minimize pain and distress

Learn more
Upcoming EventsUpcoming Events
10th World Congress on Alternatives and Animal Use in the Life Science

The 3Rs in Action
Seattle, WA
September 2017

News & Publications

June 20, 2016

CAAT offering training in Tox21 technology

The Johns Hopkins University Center for Alternatives to Animal Testing (CAAT) is offering courses on Tox21 and evidence-based toxicology in The Master of Science in Public Health (MSPH) Track in Toxicity Testing and Human Health Risk Assessment of Environmental Agents. MSPH is a professional degree program that provides individuals with the knowledge and tools needed to be at the forefront of the transition in human health risk assessment.

Click here for complete details, including funding details, part-time options, and admissions and degree requirements. There is still time to apply for fall 2016 admission.


June 13, 2016

Welfare of wild animals in research

Following a successful first event held in 2015, the RSPCA and the Animal and Plant Health Agency (APHA) are jointly convening a second one-day meeting on the welfare of wild animals in research - undertaken either in the wild or in captivity.

The meeting will take place on Thursday, 22 September 2016 in the south-east of England. The program will include presentations and discussion relating to reducing the impact of procedures in the field such as capture, trapping and tagging, and refining housing and care in captivity. It will also cover aspects of regulation and ethical review. You can register for this meeting by completing an online form.


May 24, 2016

Help with reporting animal-based research

Missing important details from publications can mean that results from animal-based studies cannot be replicated, and consequently more animals may be required to repeat the work. A new tool from Penelope assists researchers to report according to the most relevant checklist from the EQUATOR library, and hence assists implement the principle of Reduction.


May 16, 2016

Help with Experimental Design

Want to know the right number of animals to request for your particular study?

Check out the Experimental Design Assistant, a free resource from the NC3Rs to support researchers in the planning of animal experiments - ensuring robust study design and reliable and reproducible findings.


May 2, 2016

2017 Science-based Refinement Awards

Attention veterinarians, lab technicians, animal technicians, and all who work with laboratory animals: The Johns Hopkins Center for Alternatives to Animal Testing (CAAT) now is accepting proposals for the 2017 Science-based Refinement Awards.

Deadline is November 18, 2016.


Recent publications:

  • Chen M et al. (2016)
    Tattooing various combinations of ears, tail, and toes to identify mice reliably and permanently. Journal of the America Association for Laboratory Animal Science 55(2):189-198.

News Archive
Three Rs Searches Three Rs
 Searches

Three Rs Search Guide
Step-by-step strategy for Three Rs information searches

Animal Use Protocol Worksheet
Tool to organize Three Rs information for protocols

CCAC Reference Database
Database of scientific literature used in CCAC publications

Systematic Review
A method of synthesizing scientific evidence

Animal Index
Three Rs information organized by species

Testing Testing

Alternative Test Methods Table
Validated alternatives to conventional regulatory test methods