Three Rs Microsite

Your link to information on replacement,
reduction and refinement alternatives
for animal use in science

Three Rs The Three Rs

Replacing animals in science.

Reducing the number of animals used

Refining care and procedures to minimize pain and distress

Learn more
Upcoming EventsUpcoming Events
3Rs Alternatives and Consistency Testing in Vaccine Lot Release Testing

Egmon Aan Zee, The Netherlands
September 16-18, 2015

AALAS 66th National Meeting

Phoenix, AZ
November 1-5, 2015

CALAS 55th Annual Symposium

Toronto, ON
June 11-14, 2016

10th World Congress on Alternatives and Animal Use in the Life Science

The 3Rs in Action
September 2017
Seattle, WA

News & Publications

Aug 3, 2015

Read the latest issue of ALTEX.

July 27, 2015

A new strategy for achieving Three Rs impact in the assessment of toxicokinetics and systemic toxicity has been proposed for Europe.

July 20, 2015

Read this summary of research on the creation of human hepatocyte-like cells with a longer life span and the ability of these new cells to predict drug-induced liver injury.

July 13, 2015

A recent paper in PLOS Biology examines the challenges of moving away from animal models in safety testing.

Burden N., Sewell F. and Chapman K. (2015) Testing chemical safety: what Is needed to ensure the widespread application of non-animal approaches? PLoS Biol 13(5): e1002156. doi:10.1371/journal.pbio.1002156

The Three Rs microsite Alternative Test Methods Table summarizes available alternatives to animal-based regulatory test methods.

Recent publications:

News Archive
Three Rs Searches Three Rs

Three Rs Search Guide
Step-by-step strategy for Three Rs information searches

Animal Use Protocol Worksheet
Tool to organize Three Rs information for protocols

CCAC Reference Database
Database of scientific literature used in CCAC publications

Systematic Review
A method of synthesizing scientific evidence

Animal Index
Three Rs information organized by species

Testing Testing

Alternative Test Methods Table
Validated alternatives to conventional regulatory test methods